PG
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Description
Description: The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.
Also Known As:
Species: Fish
Tissue: Ovary
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.
Release Conditions: No
Depositor: Professor W Ahne, Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich, Germany
Originator: Yes
References: In Vitro 1979;15:839
Hyperlink to ECACC Cell Line Data Sheet: 95070629
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