Description: Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

Also Known As:

Species: Raccoon

Tissue: uterus

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4×10000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO2; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

Release Conditions: No

Depositor:

Originator: No

References: Proc Soc Exp Biol Med 1958;98:574

Hyperlink to ECACC Cell Line Data Sheet: 90111909