Description: Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.

Also Known As:

Species: Human

Tissue: prostate

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C.

Release Conditions: No

Depositor: Dr P Berthon, Dr O Cussenot, Hôpital SAINT-LOUIS, Paris

Originator: Yes

References: J Urol 1991;143:881; Int J Oncol 1995;6; Int J Cancer 1995;62:724, Biochem Pharm 1999;58: 279-284

Hyperlink to ECACC Cell Line Data Sheet: 95012614