PSN1
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: The human pancreatic cancer cell line PSN1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN1 cell line was established from the xenograft. PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.
Also Known As:
Species: Human
Tissue: pancreas
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3 x 10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Biochem Biophys Res Comm 1986;140:167; Cancer Res 1991;51:3356
Hyperlink to ECACC Cell Line Data Sheet: 94060601
Ask a Question
Have a question about this product? Our technical team is here to help.
