Ptk1
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Description
Description: Derived from kidney of normal adult female Potorous tridactylis in 1962, believed to be the first permanent cell line of marsupial origin to be established. Important because of low number, large size and distinct morphology of chromosomes of this mammalian species.
Also Known As:
Species: Potoroo
Tissue: kidney
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 4-5×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cytogenetics 1964;3:19; Nature 1962;194:406
Hyperlink to ECACC Cell Line Data Sheet: 91013163
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