RENE 1
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Description
Description: Derived from the endometrium tissue from the uterus of a 21-28 day old Sprague-Dawley rat. Cells carry vimentin, connexin 26 and connexin 43 markers. The transforming agent is adenovirus E1a protein.
Also Known As:
Species: Rat
Tissue: endometrium
Growth Properties: Adherent
Morphology: Cuboidal
Growth Medium: DMEM + 20µg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.
Release Conditions: No
Depositor:
Originator: No
References: Oncogene 1990;5:787
Hyperlink to ECACC Cell Line Data Sheet: 92092510
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