RENT RO3
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Description
Description: Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, uvomorulin, proenkephalin, desmin and connexin 43 markers.
Also Known As:
Species: Rat
Tissue: endometrium
Growth Properties: Adherent
Morphology: Spindle shaped
Growth Medium: DMEM + 20µg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 35 h.
Release Conditions: No
Depositor:
Originator: No
References: Oncogene 1990;5:787
Hyperlink to ECACC Cell Line Data Sheet: 92092514
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