Rolf B1.T
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Description
Description: Rolf B1.T cells arose from a spontaneous immortalisation event – retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).
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Species: Rat
Tissue: Olfactory
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 – 1:20 e.g. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr Caroline Wigley, Kings College, Guy's King's and St Thomas' School of Biomedical Sciences, Centre for Neuroscience Research, London
Originator: Yes
References: Glia 1996, 16:247-256
Hyperlink to ECACC Cell Line Data Sheet: 03071601
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