Description: RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: To keep the cells in exponential growth maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37°C; 5-7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.

Release Conditions: No

Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Originator: Yes

References: J Immunol 1967;99:839; J Biol Chem 1974;249:6812

Hyperlink to ECACC Cell Line Data Sheet: 95041316