SCP
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Description
Description: Initiated from the choroid plexus of an ovine brain. The cells support propagation of Visna virus, strain 1514 with resulting cytopathic effects. Tests have indicated that the cell line produces both detectable BVDV antigens and infectious BVDV.
Also Known As:
Species: Ovine
Tissue: brain
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J. Virol. Methods 1994; 48: 211
Hyperlink to ECACC Cell Line Data Sheet: 89101302
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