Sf 1Ep
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Description
Description: From minced ear epidermis of adult male cottontail rabbit with papillomatosis. Cells can be cultured continuously at pH 7.4 and 37°C but may also be adequately maintained at 25°C. Developed for studies of epidermis specific virus replication. Also know as Sf1Ep (NBL-11).
Also Known As:
Species: Rabbit
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan. Maximum of 80 passages expected. Cells become spindle shaped when confluent.
Release Conditions: No
Depositor:
Originator: No
References: Proc Soc Exp Biol Med 1958;98:574
Hyperlink to ECACC Cell Line Data Sheet: 90092702
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