Description: This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Also Known As:

Species: Insect

Tissue: Ovary

Growth Properties: Adherent

Morphology: Spherical

Growth Medium: TiterHigh Sf Insect Medium (Sigma I5408) no supplements.

Subculturing Procedure: Maintain cultures between 3×100,000 to 1×1,0,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.5%DMSO/92.5% 1:1 conditioned media:fresh media. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

Release Conditions: No

Depositor: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK

Originator: Yes

References: Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. 1977 The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 13(4):213-7 PMID: 68913.

Hyperlink to ECACC Cell Line Data Sheet: 05030202