Description: Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

Also Known As:

Species: Insect

Tissue: Ovary

Growth Properties: Adherent

Morphology: Spherical

Growth Medium: SF90011 + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5×10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium. This is an adherent cell line but will grow successfully in suspension. Freeze in 10% DMSO/90% foetal bovine serum (FBS). Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

Release Conditions: No

Depositor:

Originator: No

References: Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. 1977 The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 13(4):213-7 PMID: 68913.

Hyperlink to ECACC Cell Line Data Sheet: 05022801