Shmac 1
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Description
Description: Shmac 1 is an immortalised human prostate cell line which was immortalised using human papillomavirus type E6 gene from PLXSN16E6 retroviral stock (Denise Galloway, Fred Hutchinson Cancer Centre Seattle).
Also Known As:
Species: Human
Tissue: Prostate
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Release Conditions: No
Depositor: Professor. Norman J Maitland Director YCR Cancer Research Unit Department of Biology (Area 13) University of York Heslington York YO10 5DD
Originator: Yes
References: Unpublished
Hyperlink to ECACC Cell Line Data Sheet: 11102001
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