SK-N-F1
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.
Also Known As:
Species: Human
Tissue: Neural (bone marrow metastasis)
Growth Properties: Adherent/aggregates in suspension
Morphology: Epithelial-like
Growth Medium: DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4×10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Clumped cells have tendency to float.
Release Conditions: No
Depositor: Dr C Helson, St Agnes Hospital, New York
Originator: Yes
References: J Natl Cancer Inst 1984;73(1):51; Anti Cancer Drugs 1993;4:487
Hyperlink to ECACC Cell Line Data Sheet: 94092304
Ask a Question
Have a question about this product? Our technical team is here to help.
