SNU-C2B
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Description
Description: This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.
Also Known As:
Species: Human
Tissue: Cecum
Growth Properties: Semi-adherent aggregates
Morphology: Epithelial with loosely associated aggregates of cells
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate
Subculturing Procedure: Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Release Conditions: No
Depositor:
Originator: No
References: Park JG, Oie HK, Sugarbaker PH, Henslee JG, Chen TR, Johnson BE, Gazdar A: Characteristics of cell lines established from human colorectal carcinoma. Cancer Res 1987, 47(24 Pt 1):6710-6718.
Hyperlink to ECACC Cell Line Data Sheet: 96020932
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