SP2/0-Ag14
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Description
Description: Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner – in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.
Also Known As:
Species: Mouse
Tissue: unknown
Growth Properties: Suspension
Morphology:
Growth Medium: RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. HAT sensitive.
Release Conditions: No
Depositor:
Originator: No
References: Shulman M, Wilde CD, Köhler G 1978 A better cell line for making hybridomas secreting specific antibodies. Nature. 276(5685):269-70 PMID: 714156. Ozato K, Sachs DH 1981 Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 126(1):317-21 PMID: 6935293.
Hyperlink to ECACC Cell Line Data Sheet: 85072401
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