Description: STE-137 was initiated in 1963 from pooled steelhead embryos (Oncorynchus mykiss). This heteroploid cell line, also known as steelhead embryo-137, replicates and detects common salmonid viruses (infectious pancreatic necrotic virus (IPNV), infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV)). The growth temperature ranges from 15-21°C with a routine propagation at 18°C.

Also Known As:

Species: Fish

Tissue: embryo

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 18°C. Population doubling can take up to 72 hours. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Release Conditions: No

Depositor: Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis

Originator: Yes

References: In Vitro 1984;20:671

Hyperlink to ECACC Cell Line Data Sheet: 95122020