SW 620
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Description
Description: Established from the lymph node of a 51 year old Caucasian male. The cells synthesise small quantities of CEA and are highly tumorigenic in nude mice. The established cell line consists of small spherical and bipolar cells resembling microvilli.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Also Known As:
Species: Human
Tissue: colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1976;36:4562
Hyperlink to ECACC Cell Line Data Sheet: 87051203
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