T47D
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Description
Description: Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.
Also Known As:
Species: Human
Tissue: breast
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof I Keydar, Faculty of Life Sciences, University of Tel Aviv
Originator: No
References: Biochem 1981;200:315
Hyperlink to ECACC Cell Line Data Sheet: 85102201
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