Description: Peripheral blood lymphocytes from a healthy female Tunisian immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 IGHC genes: on both chromosomes 14, deletion I (del G1-EP1-A1-GP-G2-G4). The Gm-Am genotype is Gm5*; – ; – A2m2/Gm5*; – ; – A2m2 (Gm b*; – ; – A2m2/Gm b*; – ; – A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). An Epstein-Barr-transformed lymphoblastoid cell line with an identical deletion I, at the homozygous state, is available, from one member of another Tunisian family, as EZZ (TOU II-4) (93093003).

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures at 5-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof M-P Lefranc and Prof G Lefranc, Laboratoire d'ImmunoGenetique Moleculaire (LIGM), Universite de Montpellier

Originator: Yes

References: Nature 1982;300:760; Eur J Immunol 1983;13:240; FEBS Letters 1987;213:231, Immunodeficiency reviews 1991;2:265; Human Genetics 1994;93:520.

Hyperlink to ECACC Cell Line Data Sheet: 93093004