TGP 54

CODE: 94022430
Mouse pancreatic islet cell tumour

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Description

Description: TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

Also Known As:

Species: Mouse

Tissue: pancreas

Growth Properties: Adherent

Morphology:

Growth Medium: DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof OS Pettengill and Dr DS Longnecker, Dartmouth Medical School, Lebanon, NH

Originator: Yes

References: Carcinogenesis 1994;15:61

Hyperlink to ECACC Cell Line Data Sheet: 94022430

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