TGP 55
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Description
Description: TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.
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Species: Mouse
Tissue: pancreas
Growth Properties: Adherent
Morphology: Polymorph
Growth Medium: DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof OS Pettengill and Dr DS Longnecker, Dartmouth Medical School, Lebanon, NH
Originator: Yes
References: Carcinogenesis 1994;15:61
Hyperlink to ECACC Cell Line Data Sheet: 95062833
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