TK-TS 13
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Description
Description: This line is a thymidine kinase deficient mutant of a ts 13, a temperature-sensitive mutant of BHK 21. Reversion rate is very low but the cells can be reselected in BUdR.
Also Known As:
Species: Hamster
Tissue: kidney
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Mol Cell Biol 1982;2:1145; J Cell Physiol 1977;192:425
Hyperlink to ECACC Cell Line Data Sheet: 93120838
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