TK6 (IVGT)
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Description
Description: JCRBNo 1435 lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.
Also Known As:
Species: Human
Tissue: Spleen
Growth Properties: Suspension
Morphology: Small round cells in suspension
Growth Medium: RPMI1640 + 10% Horse Serum + 2mMolar L-Glutamine+ 1.8 mMolar Sodium Pyruvate. Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)
Subculturing Procedure: Dilute cell in fresh medium at 0.1- 1.5 x 105 cells per ml.
Release Conditions: No
Depositor:
Originator: No
References: Skopek TR, Liber HL, Penman BW, Thilly WG. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 1978 Sep 29;84(2):411-6. PubMed PMID: 214074
E. Lorge, M.M. Moore, J. Clements, M. O’Donovan, M.D. Fellows, M. Honma, A. Kohara, S. Galloway, M.J. Armstrong, V. Thybaud, B. Gollapudi, M.J. Aardema, J.Y. Tanir. 2016 Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing. Mutation Research. 809: 1-15.
Hyperlink to ECACC Cell Line Data Sheet: 13051501
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