Description: TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Also Known As:

Species: Mouse CD1

Tissue: Cornea

Growth Properties: Adherent

Morphology: Epithelial; small and regular cobblestone-like shape

Growth Medium: Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2 x 102 – 2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Release Conditions: No

Depositor: Dr Tetsuya Kawakita, Shinanomachi35 , Shinjuku, Tokyo, Japan 160-8583

Originator: Yes

References: Kawakita T, Shimmura S, Hornia A, Higa K, Tseng SC. 2008 Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell. J Cell Mol Med. 12(4):1303-16 PMID: 18318692.

Hyperlink to ECACC Cell Line Data Sheet: 11033107