TM3
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Description
Description: Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.
Also Known As:
Species: Mouse
Tissue: testis
Growth Properties: Adherent
Morphology:
Growth Medium: Ham’s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37²C.
Release Conditions: No
Depositor:
Originator: No
References: Bio Repro 1980;23:243; Ann NY Acad Sci 1982;383:44
Hyperlink to ECACC Cell Line Data Sheet: 91060526
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