Description: Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Also Known As:

Species: Human

Tissue: Skin (bone marrow metastasis)

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Int J Oncology 1995;7:51

Hyperlink to ECACC Cell Line Data Sheet: 94092303