WEHI 3BD
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Description
Description: Subclone of the line WEHI 3D. Cells will not respond to G-CSF or GM-CSF to differentiate in a plaque assay.
Also Known As:
Species: Mouse
Tissue: blood
Growth Properties: Semi-adherent
Morphology: Granulocyte
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-5×100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 92110401
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