WI 26 VA4
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Description
Description: An SV40 transformed derivative of WI-26, a human diploid cell line derived from embryonic lung tissue of a male Caucasian. The cells have SV40 T-Ag but infectious virus has not been rescued.
Also Known As:
Species: Human
Tissue: Lung, foetal
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Comp Physiol 1965;65:69; Exp Cell Res 1961;25:585
Hyperlink to ECACC Cell Line Data Sheet: 89101301
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