WI 38VA13 Subline 2RA

ECACC Cell Lines

CODE: 85062512

Human Caucasian foetal lung"," SV40 transformed

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Description
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Description

Description: An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

Also Known As:

Species: Human

Tissue: Lung, foetal

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Inst 1964;32:917

Hyperlink to ECACC Cell Line Data Sheet: 85062512

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