WKD (HeLa derivative)
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Description
Description: Originally derived from normal human conjunctiva. The cells support the growth of influenza A-B virus, poliovirus 1, VSV (Indiana strain) and influenza. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Soc Exp Biol Med 1954;87:440
Hyperlink to ECACC Cell Line Data Sheet: 93120839
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