XC
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Description
Description: Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Also Known As:
Species: Rat
Tissue: muscle
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nature 1960;186:980; Proc Natl Acad Sci, USA 1969;63:753; Assay: Virology 1970;42:1136
Hyperlink to ECACC Cell Line Data Sheet: 88120601
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