XrS6-hamKu80
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Description
Description: The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.
Also Known As:
Species: Hamster
Tissue: ovary
Growth Properties: Adherent
Morphology:
Growth Medium: Ham’s F12 + 2mM Glutamine + 300µg/ml G418 + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr P Jeggo, MRC Cell Mutation Unit, University of Sussex
Originator: Yes
References: Mol Cell Biol 1997;17:1264
Hyperlink to ECACC Cell Line Data Sheet: 96012301
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