YO

CODE: 85110501
Rat myeloma

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Description

Description: The full designation of this line is YB2/3.0Ag30. YO was derived from a hybrid myeloma YB2/3HL by cloning in soft agar to select a non-secreting sub-population. The cells are azaguanine resistant and do not secrete IgG.

Also Known As:

Species: Rat

Tissue: myeloma

Growth Properties: Suspension

Morphology:

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge

Originator: Yes

References: J Cell Biol 1982;93:576

Hyperlink to ECACC Cell Line Data Sheet: 85110501

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