ZR-75-30
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Description
Description: Derived from ascitic fluid of a 47 year old woman with infiltrating ductal carcinoma. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: breast
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Cells produce a significant amount of cellular debris appearing as black particles. This cell line grows relatively slowly and requires passaging approximately every 5 days.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1978;38:3352; Cancer Res 1978;38:4327
Hyperlink to ECACC Cell Line Data Sheet: 88113004
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