108CC5-TG-1
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Description
Description: A 6-thioguanine resistant mutant of 108CC5 (ECACC catalogue number 08062521)”, a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid
Also Known As:
Species: Mouse x rat hybrid
Tissue: neural
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.
Release Conditions: No
Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.
Originator: Yes
References: Heumann et al.,” (1979) Clonal hybrid cell lines expressing cholinergic and adrenergic properties. Proc. Natl. Acad. Sci. 76: 4674-4677.
Hyperlink to ECACC Cell Line Data Sheet: 08062543
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