59M
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Description
Description: Derived from a patient with carcinoma of the ovary and is of ascitic fluid origin.
Also Known As:
Species: Human
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 20 IU/l Bovine Insulin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2-1:3 i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr A Wilson, Oncology Research Laboratory, Derby City Hospital
Originator: Yes
References: Hills CA, Kelland LR, Abel G, Siracky J, Wilson AP, Harrap KR 1989 Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br J Cancer. 59(4):527-34 PMID: 2653399.
Hyperlink to ECACC Cell Line Data Sheet: 89081802
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