Description: COLO 205, COLO 201 (ECACC Catalogue 87091201) and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Also Known As:

Species: Human

Tissue: Colon

Growth Properties: Attached and Suspension

Morphology: Round and refractile cells in suspension. Some cuboidal cells in the monolayer

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain suspension cells at 3-9×100,000 cells/cm3. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3×10,000 cells/cm²; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Originator: Yes

References: Semple TU, Quinn LA, Woods LK, Moore GE 1978.Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38(5):1345-55 PMID: 565251.

Hyperlink to ECACC Cell Line Data Sheet: 87061208