Description: Subclone of MOLT-4 (ECACC catalogue no. 85011413) and is a high expresser of the cortical thymocyte differentiation antigen HTA1 and can be used for the production of CD1DmRNA.

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Cells should not remain in culture for long periods otherwise expression of CD1 is lost.

Release Conditions: No

Depositor: Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge

Originator: Yes

References: Burrone OR, Calabi F, Kefford RF, Milstein C 1983 Somatic variants of the level of expression of a cell surface antigen. EMBO J. 2(9):1591-5 PMID: 11892817.

Hyperlink to ECACC Cell Line Data Sheet: 92111101