AT-2.1
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Description
Description: AT-2.1 was developed via in vivo passages of the Dunning R-3327 rat prostatic cancers. A series of other cell lines were produced and they hold in common with Dunning R-3327 and AT-2.1 a structural change in chromosome 4 involving a translocation.
Also Known As:
Species: Rat
Tissue: ventral prostate
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4×10,000 cells/ml. Cells can be easily dislodged from the monolayer mechanically, however 0.25% trypsin or trypsin/EDTA can be used; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr J Isaacs, John Hopkins Oncology Centre, 422 N. Bond Street, Baltimore
Originator: Yes
References: The Prostate 1988;13:165
Hyperlink to ECACC Cell Line Data Sheet: 94101450
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