ATDC5
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Description
Description: The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.
Also Known As:
Species: Mouse
Tissue: Unknown
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM: Ham’s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO2; 37°C subculture every 2-3 days.
Release Conditions: No
Depositor:
Originator: No
References: Exp. Cell Res 1998; 241:1, J Bone Miner Res 1997;12:1174
Hyperlink to ECACC Cell Line Data Sheet: 99072806
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