b.End3
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Description
Description: The cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.
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Species: Mouse
Tissue: brain
Growth Properties: Adherent
Morphology: Endothelial-like
Growth Medium: DMEM + 2mM Glutamine + 5µM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof W Risau/Dr E Wagner, Department of Molecular Cell Biology, MPI for Physiological and Clinical Research, Bad Nauheim
Originator: Yes
References: Cell 1989;57:1053; Semin Cancer Biol 1994;5:137
Hyperlink to ECACC Cell Line Data Sheet: 96091929
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