Description: Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 (ECACC Catalogue number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Small rounded cells

Growth Medium: RPMI-1640 + 2mM L-Glutamine + 1% Non-Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS) + 20U/ml Interleukin-6 (IL-6).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Media change every four days. Saturation density 1.1 x 106/ml

Release Conditions: Yes – B12.F8 CANNOT be released without permission from the depositor. Please complete attached cell line release authorisation form.

Depositor: Mario U Mondelli, MD PhD FRCP, Professor of Infectious Diseases, University of Pavia, Director, Research Laboratories and Hepatology Centre, Department of Infectious Diseases, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Originator: Yes

References: Cerino A, Boender P, La Monica N, Rosa C, Habets W, Mondelli MU. 1993. A human monoclonal antibody specific for the N terminus of the hepatitis C virus nucleocapsid protein. J Immunol. 151(12):7005-15 PMID: 7505020.

Hyperlink to ECACC Cell Line Data Sheet: 11060102