B95-8
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Description
Description: Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2. In some instances the cell line B95-8 has been described as derived from marmoset, however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling.
Also Known As:
Species: Monkey
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblast/Fibroblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended that this cell line be frozen at no less than 8×1000,000 cells per ampoule.
Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1973;70:190
Hyperlink to ECACC Cell Line Data Sheet: 85011419
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