B95a
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Description
Description: B95a is a derivative of the cell line B95-8 and was adapted to growth in adherence to the substrate surface. The parent line B95-8 was derived from a cotton-top marmoset (Saguinus oedipus). The B-lymphoblastoid cells are transformed with Epstein-Barr virus (EBV). B95a has been reported to be 10,000-fold more sensitive to measles virus present in clinical specimens than Vero cells and are useful host cells for the isolation of measles virus.
Also Known As:
Species: Monkey
Tissue: Blood
Growth Properties: Semi-adherent
Morphology:
Growth Medium: DMEM + 2mM Glutamine + 5%Foetal Bovine Serum (FBS) (for growth medium) or 2% FBS (for maintenance medium)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 every 3-4 days using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; cells grow initially as a monolayer, but will pile up and detach in maintenance medium.
Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union
Depositor:
Originator: No
References: J Virol 1990: 64;700
Hyperlink to ECACC Cell Line Data Sheet: 01092505
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