BW5147.G.1.4.OUAR.1
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Description
Description: This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100µM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Also Known As:
Species: Mouse
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1974;52:429
Hyperlink to ECACC Cell Line Data Sheet: 88100507
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