Description: CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Also Known As:

Species: Mouse

Tissue: Embryo

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Subculturing Procedure: These cells may take 1-2 days to attach and will initially grow in loosely attached small islands. The islands will increase in size in the following 1-2 days and should be sub-cultured when the flask reaches 70-80% confluence. Cells should be removed by gentle knocking of the flask and pipetting. Split cultures 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² ;5% CO₂; 37°C.

Release Conditions: No

Depositor: Dr D. Chikaraishi, Dept of Neurobiology, 427G Bryan Research Building, Duke University Medical Centre, Durham, North Carolina USA 27710

Originator: Yes

References: Suri et al.,” (1993) Catecholaminergic Cell lines from the Brain and Adrenal Glands of Tyrosine Hydroxylase-SV40 T Antigen Transgenic Mice. Journal of Neuroscience 13: 1280-1291 PMID: 7680068

Hyperlink to ECACC Cell Line Data Sheet: 08100805