CHO-K1.Cl6
CHO-K1", Fc gamma RIIIa , Fc gamma receptor 3A, 158V, allotype, human IgG ," IgG binding
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Description
Description: Clone of CHO-K1 cells expressing human FcgammaRIIIa of the 158V allotype anchored to glycosyl phosphatidylinositol
Also Known As:
Species: Hamster
Tissue: Ovary
Growth Properties: Adherent
Morphology: Polygonal
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10000 cells/cm2 using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37°C
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form
Depositor: Dr Kathryn L Armour
Originator: Yes
References: Armour KL, Smith CS, Clark MR. Expression of human Fc gamma RIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding. J Immunol Methods. 2010 Mar 31;354 (1-2):20-33. doi: 10.1016/j.jim.2010.01.011. Epub 2010 Feb 4. PMID:20138184 [PubMed – indexed for MEDLINE
Hyperlink to ECACC Cell Line Data Sheet: 15042901
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