CHO-WBL (IVGT)
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Description
Description: The Chinese hamster ovary CHO cells were originally established as spontaneously transformed cells from an ovarian biopsy by Tijo and Puck (see 1958 references below).
Also Known As:
Species: Hamster
Tissue: Ovary
Growth Properties: Adherent
Morphology: Polygonal
Growth Medium: McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Pipette to break up clumps.
Release Conditions: None
Depositor:
Originator: No
References: Tjio JH Puck TT.1958 Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med.108 (2):259-68. PMID:13563760.
Puck TT, Cieciura SJ, Robinson A. 1958 Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med.108 (6):945-56. PMID: 13598821.
E. Lorge, M.M. Moore, J. Clements, M. O’Donovan, M.D. Fellows, M. Honma, A. Kohara, S. Galloway, M.J. Armstrong, V. Thybaud, B. Gollapudi, M.J. Aardema, J.Y. Tanir. 2016 Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing. Mutation Research. 809: 1-15.
Hyperlink to ECACC Cell Line Data Sheet: 14043001
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